The cururu toad (Rhinella jimi) is an anuran belonging to the fauna of the Brazilian northeast region. Brief information are known about the composition of the secretion released from your parotoid glands. First report on the ionome of the secretion of the parotoid glands of R. jimi, investigating the influences of abiotic factors such as biome, seasonality and gender. ICP-MS was used for measurements combined with principal component analysis (PCA). A screening of the secretion sample detected 68 elements which the total concentration of 18 elements was determined. PCA revealed that biome and seasonality factors have a greater influence on the ionomic profile of parotoid secretion. The presence of toxic metals in the secretion samples indicates that the cururu toad can be considered a potential bioindicator. These findings may contribute to understanding the metabolism, lifestyle and interaction of the cururu toad with environmental factors as well as open new perspectives to investigate the relationships of the ionome with other biomolecules, for example, metalloproteins and their physiological functions.