Banca de QUALIFICAÇÃO: IRAKERLEY ALVES FERNANDES

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
DISCENTE: IRAKERLEY ALVES FERNANDES
DATA: 28/09/2026
HORA: 08:00
LOCAL: Sala de Aula 2 - PPGQ
TÍTULO: Optimization of homogenic liquid-liquid microextraction using switchable hydrophilicity solvent for the extraction of β-carotene in biofortified sweet potatoes and cassava
PALAVRAS-CHAVES: Switchable hydrophilicity solvent, microextraction, cassava sweet potato, and β-carotene
PÁGINAS: 26
GRANDE ÁREA: Ciências Exatas e da Terra
ÁREA: Química
SUBÁREA: Química Analítica
ESPECIALIDADE: Separação
RESUMO:

β-Carotene is a naturally occurring, bioactive compound found in various fruits and vegetables. Determining its concentration in foods is of great nutritional importance; however, it is challenging due to matrix interference. In this study, A simple, rapid, and sensitive method of homogeneous liquid-liquid microextraction using a switchable hydrophilicity solvent (SHS-HLLME) was optimized for the extraction and determination of β-carotene in complex food matrices (sweet potato and cassava genotypes) prior to HPLC-DAD analysis. The optimal extraction conditions consisted of using N,N-dimethylcyclohexylamine (DMCHA) as the switchable hydrophilicity solvent (SHS), 1000 μL of a mixture of SHS: 6 mol L⁻¹ HCl (1:1 v/v), 600 μL of 10 mol L⁻¹ NaOH, and 1000 μL of 2.0% (w/v) NaCl. Under the optimized conditions, the proposed method exhibited a linear range of 1–40 µg L⁻¹, with limits of detection and quantification of 0.305 µg L⁻¹ and 0.926 µg L⁻¹, respectively. The intra-day and inter-day precision of the developed method yielded RSD values in the range of 1.1–3.7% and 1.2–3.6%, respectively. The recovery rates for β-carotene extraction ranged from 97.9% to 107.0%, with RSD values less than 5%. The determination of β-carotene in real samples was performed in just 3.5 minutes using the proposed methodology. The results demonstrated that the SHS-HLLME approach is fast, simple, eco-friendly, and provides satisfactory analytical performance.

MEMBROS DA BANCA:
Presidente - 1635927 - EDIVAN CARVALHO VIEIRA
Interno - 2074119 - ANTÔNIA LAÍRES DA SILVA SANTOS
Interno - 2350685 - BENEDITO BATISTA FARIAS FILHO
Interno - 1998602 - EVERSON THIAGO SANTOS GERONCIO DA SILVA
Interno - 1210376 - SAMYA DANIELLE LIMA DE FREITAS
Externo ao Programa - 676.***.***-49 - VALDILEIA TEIXEIRA UCHOA - UESPI