Banca de QUALIFICAÇÃO: EVALDO DOS SANTOS MONÇÃO FILHO

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
DISCENTE: EVALDO DOS SANTOS MONÇÃO FILHO
DATA: 23/09/2025
HORA: 08:30
LOCAL: Auditório do PPGQ
TÍTULO: Untargeted metabolomics molecular networking, cytotoxic and antiplasmodial activities of Combretum lanceolatum (Pohl ex. Eichler) leaves
PALAVRAS-CHAVES: Combretaceae, GNPS, Molecular networking, Malaria
PÁGINAS: 50
GRANDE ÁREA: Ciências Exatas e da Terra
ÁREA: Química
SUBÁREA: Química Orgânica
ESPECIALIDADE: Química dos Produtos Naturais
RESUMO:

Combretum genus is recognized for its high pharmacological potential and applications. This study aimed the chemical characterization and evaluate the cytotoxic and antiplasmodial activities of C. lanceolatum leaves. The ethanolic extract (EECLL) was prepared and the hexane (HF), diethyl ether (EEF), ethyl acetate (EAF), hydromethanolic (HMF) and precipitate (PP) fractions were obtained by liquid-liquid extraction. The HF was analysed by gas chromatography-mass spectrometry possibiliting the annotation of 27 compounds of different classes such as fatty acids and their derivatives, tocotrienols and terpenoids. Furthermore, a mix of stigmasterol and β-sitosterol (24+25), (E)-phytol (28) and 3,7,11,16-tetramethyl-hexadecan-1,2-diol (29) were isolated for the first time in C. lanceolatum species from HF fraction. The EECLL and EEF, EAF, HMF and PP fractions were analysed by ultra performance liquid chromatography coupled to mass spectrometry and set in the Global Natural Products Social Molecular Networking (GNPS) web platform, made possible the annotation of 28 compounds, such as fatty acids, triterpenoids, flavonoids and other phenolic compounds. The EECLL, fractions and compounds 24+25 and 28 were tested for antiplasmodial activity against P. falciparum W2 resistant strains, all tested samples presented activity (Except HMF and compound 28). The HF showed the best IC₅₀ 6.83 ± 1.56 mg/mL compared to other tested samples, and lower than the positive control chloroquine (IC₅₀ 0.53 ± 0.07 mg/mL), demonstrating the most promising antimalarial potential. None of the extract and fractions presented cytotoxic activity against tumoral or non-tumoral cells lines, indicating low toxicity. This study revealed that C. lanceolatum leaves is rich in secondary metabolites, with the untargeted metabolomic issues applied for the first time and demonstrating a good biological potential for further application.

MEMBROS DA BANCA:
Externo à Instituição - 379.***.***-09 - ANA CAROLINE ZANATTA SILVA - FCFRP-USP
Presidente - 1888044 - GERARDO MAGELA VIEIRA JUNIOR
Externo ao Programa - 1731422 - JOAO SAMMY NERY DE SOUZA
Interno - 1614544 - JURANDY DO NASCIMENTO SILVA
Externo à Instituição - 055.***.***-37 - WEIDER HENRIQUE PINHEIRO PAZ - UFAM